Mechanisms of oxidative biomolecular interaction

Our approach is to study biomolecular interactions between peptide models and biomimetic soft matter, under oxidative stress conditions, in the overall context of neurodegenerative diseases.

The hyperphosphorylation and subsequent aggregation of Tau, a protein associated with microtubules, are involved in the formation of neurofibrillary tangles in Alzheimer's disease. The progressive accumulation of oligomers of the β-amyloid peptide in the brain is also a marker of this pathology. Despite the established link between oxidative stress and Alzheimer's disease, the direct oxidation of the Tau protein and the peptide Aβ and their consequences are poorly documented.

In this context, we are interested in:
> the oxidation mechanisms at the molecular level,
> the oxidative interactions with organized systems (unilamellar vesicles, bicelles, biomimetic models of microtubules, etc.),
> the consequences of oxidation on cell viability,
> the metabolome response.

> Multidisciplinary analysis of protein-lipid interactions and implications in neurodegenerative disorders
Collin F.; Cerlati O.; Couderc F.; Lonetti B.; Marty J-D.; Mingotaud A-F.
Trends Anal. Chem., 2020, 132, 116059, DOI

> Oxidative stress and the amyloid beta peptide in Alzheimer’s disease
Cheignon C.; Tomas M.; Bonnefont-Rousselot D.; Faller P.;  Hureau C.; Collin F.
Redox Biol., 2018, 14, 450-464, DOI