Laboratoire des Interactions Moléculaires et Réactivité Chimique et Photochimique
UMR 5623

Camille Courtine, PhD student at IMRCP, has defended her thesis on a photosensitive hydrogel

Camille carried out her research in the IDeAS team at the IMRCP laboratory.
On 10th February, she defended her thesis entitled: "Macro and supramolecular hydrogels responsive to visible light"

In biological environment, cells interact with their surroundings, the properties of which strongly influence their behaviour. Nowadays, studying the relationship between mechanical stress and cell fate is the key to understand many pathologies evolutions such as cancer.
Hydrogels are materials whose properties can be tuned to mimic the cell environment. The objective of this PhD project, funded by the National Research Agency (ANR), was to develop hydrogels with mechanical properties photocontrolable with visible light.

The purpose of this gel was to become a sample carrier to study how different biological tissues respond to the change in the mechanical properties of their surroundings.
Hydrogels based on three water soluble building blocks were designed. They were composed of two intertwined chemical and physical networks. Those hydrogels were characterized by techniques such as scanning electron microscopy, mechanical compression testing and atomic force microscopy.

The results showed that the gels had stimulable photo-chemical properties and that their mechanical properties were tunable, depending on the formulation used, with Young's moduli between 5 and 25 kPa, compatible with a range of biological tissues.

Besides, experiment with biological samples shed light of their biocompatibility with cardiomyoblasts. These transparent materials especially enabled the imaging of an encapsulated living tissues with confocal microscopy.

 

Top left: a swollen hydrogel ©Camille Courtine / IMRCP
Top right: cryo-SEM image of the microstructure of the hydrogels ©Bruno Payré / CMEAB
Bottom left: a hydrogel mounted of the compression testing cell ©Camille Courtine / IMRCP
Bottom right: mouse biopsy encapsulated into a hydrogel and observed through confocal microscopy ©Nicolas Pataluch / I2MC

 

Highlight of the thesis:

  • these results were published in the Journal of Organic Chemistry;
  • Camille presented her work at the international NICE conference on Bio-inspiration & Biobased Materials in October 2020.

Congratulations to Camille for the quality of her work!

2022 IMRCP