Laboratoire des Interactions Moléculaires et Réactivité Chimique et Photochimique
UMR 5623

Séminaire par Dr Patrice Castignolles / Western Sydney University

Lundi 12 février 2018 - 14h - salle Maroni / MHT

Patrice Castignolles, chercheur à l'université de Western Sydney, fera une présentation sur la : Capillary electrophoresis: a new hope in characterisation of the composition and structure of polyelectrolytes and in monitoring their chemical reactions

We propose free solution capillary electrophoresis (CE) as a method to characterise polyelectrolytes such as double hydrophilic block copolymers, poly(acrylic acid) (PAA), chitosan but also starch, poly(N-vinyl pyrrolidone) or PEG. The separation by CE is not influenced by the degree of polymerisation (or size for homopolymers) and is thus in the critical conditions. We will show how distributions of compositions can be determined for copolymers.[1,2] We will also show that the CE technique is robust, especially no sample filtration is required. Reactions can thus be monitored online. We will show the examples of monitoring the binding of cisplatin to PAA, grafting of peptides or PEG on chitosan films[3, 4] or monitoring sugars in a polysaccharide digestion[5].


[1] JJ Thevarajah, MP Van Leeuwen, H Cottet, P Castignolles, M Gaborieau, Determination of the Distributions of Degrees of Acetylation of Chitosan. International Journal of Biological Macromolecules 2017, 95, 40-48.
[2] JJ Thevarajah, AT Sutton, AR Maniego, EG Whitty, S Harrisson, H Cottet, P Castignolles, M Gaborieau, Quantifying the heterogeneity of chemical structures in complex charged polymers through the dispersity of their distributions of electrophoretic mobilities or of compositions. Analytical Chemistry 2016, 88, 1674-1681.
[3] JJ Thevarajah, MD O'Connor, P Castignolles, M Gaborieau, Capillary Electrophoresis to Monitor Peptide Grafting onto Chitosan Films in Real Time. Journal of Visualized Experiments 2016, e54549.
[4] DL Taylor, JJ Thevarajah, DK Narayan, P Murphy, MM Mangala, S Lim, R Wuhrer, C Lefay, MD O'Connor, M Gaborieau, P Castignolles, Real-time monitoring of peptide grafting onto chitosan films using capillary electrophoresis. Analytical and Bioanalytical Chemistry 2015, 407, 2543-2555.
[5] JD Oliver, AT Sutton, N Karu, M Phillips, J Markham, P Peiris, EF Hilder, P Castignolles, Simple and robust monitoring of ethanol fermentations by capillary electrophoresis. Biotechnology and Applied Biochemistry 2015, 62, 329-342.


2018 IMRCP