Séminaire par Dr Cédric Montanier / LISBP
Cédric Montanier, chercheur au Laboratoire d''Ingénierie des Systèmes Biologiques et des Procédés à Toulouse, fera une présentation sur : Investigating spatial proximity in plant cell wall multi-modular degrading enzymes
In Nature, the plant-based organic carbon contained within plant cell walls is mainly recycled by the action of cellulolytic microorganisms, such as bacteria and fungi, which produce complex arrays of cell wall-degrading enzymes. Many of the enzymes that degrade plant cell wall polysaccharides are modular proteins, which contain single or multiple copies of both catalytic domain(s) and CBM(s). Cellulolytic microorganisms developed a couple of strategies to break down the complex plant cell wall; secrete free enzymes into their surroundings or produce large multi-component cell-bound structures, which harbour several enzymes displaying complementary activities. In both case, substrate is attacked by different enzymes acting together at different regions, and much more than sum of individual different enzymatic activities, synergism drives the efficiency of such system. However, spatial proximity between enzymes and its impact over synergism in enzymatic cascade is rarely investigated. The seminar will focus on several approaches to investigate these enzymes at work.
